Aim
I am going to investigate how the temperature affects the catalyse enzyme on heat content peroxide, using potato discs for the catalyse.
Hypothesis
Catalyse is a biological enzyme which works outmatch between 30 and 40 degrees, kindred most enzymes. It too has an optimum pH.
Catalyse breaks down Hydrogen Peroxide into water and oxygen. instal and animal cells contain catalyse.
`I foretell that the catalyse enzyme instal in the potato allow for break down the substrate (Hydrogen Peroxide) quickest at 37- 40 degrees Celsius because enzymes work best at certain temperatures, usually body temperature. If the temperature is above 40 degrees the enzyme will become denatured so molecules of Hydrogen Peroxide wont be able to fit into it and so they cant form an enzyme substrate complex and cant be broken down, so no oxygen or water is created. If it is below 37 degrees the amount of activity is reduced, as temperature is a limiting factor in this part of the experiment.
I predict that the graph for the total oxygen evolved will look like this:
I think this because between A and B the rate of response increases the molecules of Hydrogen Peroxide will have to a greater extent energising energy and so there will be more than collisions, as they move around the container and hit individually other. more(prenominal) enzyme substrate complexes will be formed and so more oxygen and water is formed.
At point B the temperature is the optimum.
Between points B and C the temperature is too high for the enzyme to work and the shape of the active post is damaged. The reaction slows down, as the enzyme is eventually denatured.
Equipment
Stop clock
10ml measuring cylinder
Delivery tubes
Bung
Googles
Hydrogen peroxide (...ml each time)
Potato discs (40 1mm thick)
Controls
Amount of hydrogen peroxide (20ml)
Length...
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